iQue Forecyt® Reference Guide (Version 10.0)

Mixing Samples with the iQue® Shaker

 

iQue® Screener, iQue® Screener PLUS and the iQue® 3

Plate Type	Well Volume	MAX rpm
96-well	20-40µL	2,600
96-well	40-60µL	2,200
96-well	60+ µL	A/O*
384-well	10-30 µL	3,000
384-well	30-50 µL	2,800
384-well	50+ µL	A/O*

 

iQue® Screener HD

Plate Type	Well Volume	MAX rpm
96-well	20-40µL	3,200
96-well	40-60µL	2,400
96-well	60+ µL	A/ O*
384-well	10-30 µL	3,500
384-well	30-50 µL	3,100
384-well	50+ µL	A/ O*
1536-well	Up to 6 µL	5,000

 

A/ O* Additional Optimization necessary. While it is possible to run these volumes, they were not routinely tested during assay development. To determine ideal shake speeds for high volume assays, iQue® recommends starting at low RPM values and slowly increasing to higher values.