iQue® Immune Cell-Based Kits enable the analysis of multiple immunological cell phenotypes and functional traits such as viability, proliferation, and cytokine release. Most of these kits are optimized for low-wash protocols. Each of these are available in different sizes to meet your screening needs. The Forecyt® software has automated templates for most of these kits making it easy to acquire and analyze data. All have been optimized for use on the iQue® Screener platforms.
iQue® Human T Cell Activation Kit: Single wash assay provides a multiplexed approach to measure T-cell activation, proliferation and quantify cytokine release in a single well. Learn More
iQue® Human T Cell Companion Kits: Simultaneously measure additional cytokines besides those provided in T Cell Immunology Kit. Learn More
iQue® Human T Cell Memory Kit: Analyze T cell memory phenotype and function at different stages with the new T Cell Memory Cell and Cytokine Profiling Kit Learn More
iQue® Human General Immune Cell Killing Kit: Evaluate cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. Learn More
iQue® Human T Cell Killing Kit: Evaluate T cell immunophenotypes and function, cell health, cell count, secreted pro-inflammatory cytokines in a single assay Learn More
iQue® Mouse T Cell Kit : Evaluate T cell activation, memory, exhaustion and cytokine secretion in the same well Learn More
iQue® Human T Cell Exhaustion Kit: Multiplex T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay Learn More
iQue® Human NK Cell Killing Kit: Measure activation markers, cell membrane integrity, cell count, secreted pro-inflammatory cytokines, and pro-apoptotic protease from a single assay Learn More
iQue®️ Human T Cell Phenotyping Kit: Evaluate phenotype and function of multiple of T subsets in the same well Learn More
The iQue® Human T Cell Phenotyping Kit (CD3, CD4 and CD8) is designed for reliable identification of human T cell subsets. This assay is optimized to run on the iQue® 3 (VBR and VYB configurations) platform combining high throughput sampling, flow cytometry detection and multiplexing capabilities.
Phenotyping analysis of human PBMCs cultured for one day. A. Set gate for live cells in all events to exclude debris/dead cells. B. Remove doublets from live cells to obtain single cells. C. CD3+ cells of Singlets. D. CD4+ and CD8+ subsets of CD3+ cells.
iQue®️ Human T Cell Phenotyping Kit (CD3, CD4 and CD8) | |
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Platform | Compatible with iQue3/iQue Screener PLUS VBR/VYB configurations |
Available Sizes | Catalog Numbers |
1 x 96 well | 97099 |
5 x 96 wells | 97100 |
1 x 384 wells | 97101 |
5 x 384 wells | 97102 |
The iQue® Human T Cell Activation Cell and Cytokine Profiling Kit assesses the activation status of T cells while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
iQue® Human T Cell Activation Kit | |
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Platform | Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration |
Available Sizes | Catalog Numbers |
1 x 96 well | 90560 |
5 x 96 wells | 90561 |
1 x 384 wells | 90562 |
5 x 384 wells | 90563 |
The iQue® Human T Cell Companion Kits can be used to measure additional cytokines besides those already included in the iQue® Human T Cell Activation Cell and Cytokine Profiling Kit or other T Cell Immunology Kits. Below are the list of available cytokines for T Cell Companion Kits.
iQue® Human T Cell Companion Kits | |
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Catalog no. | Description |
97028 | iQue® Human IL-2 T Cell Companion Kit |
97029 | iQue® Human IL-6 T Cell Companion Kit |
97030 | iQue® Human IL-10 T Cell Companion Kit |
97031 | iQue® Human IL-13 T Cell Companion Kit |
97032 | iQue® Human IL-17A T Cell Companion Kit |
97033 | iQue® Human GM-CSF T Cell Companion Kit |
97034 | iQue® Human TNF T Cell Companion Kit |
The iQue® Human T Cell Memory Kit measures T cell memory phenotype and function at different stages while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
iQue® Human T Cell Memory Kit | ||
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Platform | Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration | |
Available Sizes | Catalog Numbers | Order Now |
1 x 96 well | 97035 | iQue® Human T Cell Memory Kit |
5 x 96 wells | 97036 | |
1 x 384 wells | 97037 | |
5 x 384 wells | 97038 |
The iQue®️ T Cell Companion Kits can be used to measure additional cytokines besides those already included in the iQue®️ T Cell Memory Cell and Cytokine Profiling Kit or other T Cell Immunology Kits. Below are the list of available cytokines for T Cell Companion Kits.
iQue® Human T Cell Memory Companion Kits | |
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Catalog no. | Description |
97028 | Human IL-2 T Cell Companion Kit |
97029 | Human IL-6 T Cell Companion Kit |
97031 | Human IL-13 T Cell Companion Kit |
97032 | Human IL-17A T Cell Companion Kit |
97033 | Human GM-CSF T Cell Companion Kit |
97034 | Human TNF T Cell Companion Kit |
The iQue® Human T Cell Exhaustion Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:
iQue® Human T Cell Exhaustion Kit | |
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Platform | Compatible with iQue3/iQue Screener PLUS - VBR configuration |
Available Sizes | Catalog Numbers |
1 x 96 well | 97069 |
5 x 96 wells | 97070 |
1 x 384 wells | 97071 |
5 x 384 wells | 97072 |
The iQue® Mouse T Cell Kit measures T cell activation, memory, exhaustion while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
iQue® Mouse T Cell Kit | |
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Platform | Compatible with iQue3/iQue Screener PLUS VBR configuration |
Available Sizes | Catalog Numbers |
1 x 96 well | 97042 |
5 x 96 wells | 97043 |
1 x 384 wells | 97044 |
5 x 384 wells | 97045 |
The iQue® Human General Immune Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
iQue® Human General Immune Cell Killing Kit | |
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Platform | Compatible with iQue3/iQue Screener PLUS VBR and BR configuration |
Available Sizes | Catalog Numbers |
1 x 96 well | 97076 |
5 x 96 wells | 97077 |
1 x 384 wells | 97078 |
5 x 384 wells | 97079 |
The iQue® Human T Cell Killing Kit (CD3, CD4, and CD8) is designed for reliable identification of human T cell subsets. This assay is optimized to run on the iQue® 3 (VBR and VYB configurations) platform combining high throughput sampling, flow cytometry detection, and multiplexing capabilities.
iQue® Human T Cell Killing Kit | ||
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Platform | Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration | |
Available Sizes | Catalog Numbers | Order Now |
1 x 96 well | 97060 | iQue® Human T Cell Killing Kit |
5 x 96 wells | 97061 | |
1 x 384 wells | 97062 | |
5 x 384 wells | 97063 |
The iQue® Human NK Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:
iQue® Human NK Cell Killing Kit | |
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Platform | Compatible with iQue3/iQue Screener PLUS VBR configuration |
Available Sizes | Catalog Numbers |
1 x 96 well | 97082 |
5 x 96 wells | 97083 |
1 x 384 wells | 97084 |
5 x 384 wells | 97085 |
The iQue® Human NK Cell Companion Kits may be used in combination with the iQue® Human NK Cell Killing Kit to allow measurement of up to 6 additional human cytokines/effector proteins along with IFNγ and Granzyme B which are already included in the iQue® Human NK Cell Killing Kit.
iQue® Human NK Cell Companion Kits | |
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Catalog No. | Description |
97089 | Human MIP 1α NK Cell Companion Kit |
97090 | Human RANTES NK Cell Companion Kit |
97091 | Human GM-CSF NK Cell Companion Kit |
97092 | Human CD178/Fas Ligand NK Cell Companion Kit |
97093 | Human Granzyme A NK Cell Companion Kit |
97094 | Human TNFα NK Cell Companion Kit |
The iQue® Human Antibody Dependent Cellular Phagocytosis (ADCP) Kit enables high throughput identification of tumor elimination mediated by mAbs. The kit is designed for homogeneous labeling of target cancer cells using a green fluorescent dye, effector cell identification via (non-adherent or adherent) CD14+ labeling, as well as quantification of cell membrane integrity.
Post labeling of target and effector cells, customer test antibodies are added, inducing tumor clearance via their binding to encoded target cells and subsequent CD14+ effector cell binding and engulfment. Test antibodies bind to target cells through its Fab region and CD14+ effector cells binding via Fc region.
The readout measures the number of CD14+ effector cells that are positive for the cell encoder dye. The CD14+ effector cells can only be positive for the cell encoder dye if they have phagocytosed target cells.
The iQue® Human ADCP Kit has been validated for use on the iQue® platform with BR and VBR configurations.
iQue® Human Antibody Dependent Cellular Phagocytosis (ADCP) Kit | |
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Platform | Compatible with iQue® 3/iQue® Screener Plus - VBR and BR Configurations |
Available Sizes | Catalog Numbers |
1 x 96 well | BA-97106 |
5 x 96 wells | BA-97107 |
1 x 384 wells | BA-97108 |
5 x 384 wells | BA-97109 |