Immune Cell-Based Kits

Gain greater biological insight of activated T cells with the new T Cell Activation Cell and Cytokine Profiling Kit

Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 15 endpoints from a single well assay measuring T cell phenotypes, early and late cell activation markers in each phenotype, cell viability, cell count, proliferation and secreted effector cytokines.
• Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
• Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The T Cell Activation Cell and Cytokine Profiling Kit assesses the activation status of T cells while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
• The T cell phenotypes are profiled for the expression of 3 activation markers, CD69 (early), CD25 (late), and HLA-DR (even later, with strong stimulation).
• Optimized workflow enables the measurement of effector cytokines secreted by activated T cells, Th1 cytokine IFNγ/ multifunctional cytokine TNFα in the same assay well.

Workflow

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Intellicyt® T Cell Companion Kits

The Intellicyt® T Cell Companion Kits can be used to measure additional cytokines besides those already included in the Intellicyt® T Cell Activation Cell and Cytokine Profiling Kit or other T Cell Immunology Kits. Below are the list of available cytokines for T Cell Companion Kits.

Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 11 endpoints from a single well assay measuring T cell memory phenotype and function at different stages, cell viability, cell count, and secreted pro/anti-inflammatory cytokines.
• Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
• Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The Human T Cell Memory Cell and Cytokine Profiling Kit measures T cell memory phenotype and function at different stages while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
• The panel also includes 5 different T cell surface markers for T naive/memory/effector cell phenotyping: CD45RA, CD45RO, CD27 (long term survival), CD62L, and CD95.
• Optimized workflow enables the measurement of effector cytokines secreted by activated T cells, including pro-inflammatory cytokine IFNγ and anti-inflammatory cytokine IL-10 in the same assay well.

Workflow

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Intellicyt® T Cell Memory Cell and Cytokine Companion Kits

The Intellicyt®️ T Cell Companion Kits can be used to measure additional cytokines besides those already included in the Intellicyt®️ T Cell Memory Cell and Cytokine Profiling Kit or other T Cell Immunology Kits. Below are the list of available cytokines for T Cell Companion Kits.

Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 11 endpoints from a single-well assay measuring T cell exhaustion phenotypes and function, as well as cytokine secretion in response to stimulation, cell viability, and an optional proliferation dye allows screening for molecules that reverse T cell exhaustion.
• Biological insight: Generate functional data and analyze cell health from the same well by multiplexing cell and cytokine detection in one assay.
• Simplicity: Single platform assay and data acquisition and analysis eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The Human T Cell Exhaustion Cell and Cytokine Profiling Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:

• Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.
• The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.
• Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.
• The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).

Workflow

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Key Benefits

• Speed: Sample to actionable data by allowing multiple parameters to be assessed in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 11 endpoints from a single well assay measuring T cell activation, memory, exhaustion, cell viability, cell proliferation, and secreted pro/anti-inflammatory cytokines in mouse.
• Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
• Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The Mouse T Cell and Cytokine Profiling Kit measures T cell activation, memory, exhaustion while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
• The panel also includes 4 different T cell surface markers for T cell activation, memory and exhaustion in mouse.
• Optimized workflow enables the measurement of effector cytokines secreted by T cells, including pro-inflammatory cytokine IFNγ and anti-inflammatory cytokine IL-2 in the same assay well.

Workflow

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Key Benefits

• Speed: Expedient sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
• Results: Five endpoints from a single well assay measure target and effector cells, mitochondrial membrane depolarization, cell membrane integrity, cell count, and secreted pro-apoptotic protease Granzyme B.
• Biological insights: Investigation of multiple mechanisms of immune cell mediated cell killing in one well.
• Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.

Kit Description

The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye and mitochondrial membrane potential dye.
• Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
• Simultaneous analysis of multiple mechanisms of immune cell mediated cell killing in a single, high content assay including mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, and pro-apoptotic protease Granzyme B.

Workflow

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Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 8 endpoints from a single well assay measuring markers of T cell immunophenotypes and function, cell health, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic serine protease Granzyme B.
• Biological insight: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
• Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The Human T Cell Mediated Killing Kit was designed for ease of use in multiplexing cell phenotype and function markers along with bead-based, secreted protein profile measurements in the same assay. This one wash assay requires minimal hands on time to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD8+ T cytotoxic cells, and CD3+ CD8- T helper cells.
• The panel also includes 2 different T cell functional markers, CD25 and PD-1.
• Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Workflow

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Key Benefits

• Speed: Sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
• Results: Nine endpoints from a single well assay measuring activation markers (CD69 and CD25), cell membrane integrity, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic protease Granzyme B.
• Biological insights: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
• Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.
• Flexibility: Choose additional cytokine measurements from a validated list of Human NK Cell Companion Kits.

Kit Description

The Human NK Cell Mediated Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
• Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
• NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells. The expression of the IgG Fc receptor III (CD16) on the NK cells can then be assessed.
• The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
• Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Workflow

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Intellicyt® Human NK Cell Companion Kits

The Human NK Cell Companion Kits may be used in combination with the Human NK Cell Mediated Killing Kit to allow measurement of up to 6 additional human cytokines/effector proteins along with IFNγ and Granzyme B which are already included in the Human NK Cell Mediated Killing Kit.