Validated iQue® Cell and Bead-Based Kits allow you to get actionable data from less sample, all in a fraction of the time of conventional assays. These unique competition assay kits are no-wash, no-dilution to provide a streamlined workflow with minimal hands-on preparation.
Available iQue® Cell and Bead-Based Kits include:
iQue® Human IgG Titer & Viability Kit: The only multiplexed assay to enable simultaneous reporting of IgG quantity, cell number and cell viability in each well of cell line production screening plates. These metrics enable the precise calculation of the IgG quantity per cell and per viable cell to profile cell productivity. Learn More »
iQue® Mouse IgG Type and Titer Assay: Multiplex, high throughput, no-wash assay that enables the simultaneous measurement of 4 mouse IgG isotypes, the mouse IgG quantity for each isotype, cell number and cell viability from each well of the screening plates. Learn More »
The ONLY solution that correlates IgG quantitation per cell with cell health and growth readouts in a single, cost-effective competition assay using IgG-capture beads, IgG fluorescent competing antibody, and a cell viability stain.
Clone productivity ranking with more relevant data—Balancing IgG concentration, cell density, IgG quantitation per cell, and cell viability are crucial to selecting the optimal clones.
|iQue® Human IgG Titer & Viability Kit|
|Reagents||Human FITC-IgG||FL4 Cell Membrane Integrity Dye|
|Detection Channel||iQue Screener||FL1||FL4|
|iQue Screener PLUS||BL1||RL1|
|Available Sizes||Catalog Number|
|1 x 384 well||91142|
|5 x 384 wells||91143|
|20 x 384 wells||91144|
|50 x 384 wells||91145|
The Mouse IgG Type and Titer kit is a multiplexed, competition formatted assay to identify and quantify mouse IgG1, IgG2a, IgG2b, and IgG3. It is a no wash, no sample dilution assay that measures both cells and beads and provides these significant advantages over traditional methods:
Streamlined workflow means experiment set-up and data acquisition for this multiplex assay takes about 100 minutes—and that includes incubation!
iQue® Mouse IgG Type and Titer Workflow. Samples from hybridoma or B-cell cloning plates are directly transferred to the assay plate without an intermediate dilution step. Each assay well is seeded with four different types of capture beads, each with a specific affinity for a single IgG isotype. The IgG isotype in the sample will compete with the same FITC-labeled IgG isotype to bind to the capture bead. The IgG isotype quantity will be calculated from the isotype-specific standard curve. If cells are included in the assay well, a membrane integrity dye in the detection reagent will stain the membrane-compromised dead cells by DNA intercalation. After the addition of the detection reagent mixture and the capture beads to the samples and 60 minute incubation, the samples are acquired on the iQue Screener PLUS.
|iQue® Mouse IgG Type and Titer Assay|
|Platform||Compatible with HTFC, iQue Screener, and iQue Screener PLUS|
|Available Sizes||Catalog Numbers|
|1 x 96 well||91168|
|1 x 384 wells||91165|
|5 x 384 wells||91166|