MultiCyt Cell Cycle Kit

Key Benefits

  • Uses a DNA-specific dye so there is no need to use RNase.
  • The dye crosses membranes so there is no need to permeabilize or fix the cells. This also means you can use live cells.
  • No wash needed, simply add and read for fast, easily automated workflow.
  • Multiplex with other MultiCyt kits like Cell Membrane Integrity for richer content.

Kit Description

The Cell Cycle Kit utilizes a DNA binding dye to enable the creation of a cell cycle profile and the quantification of the relative percentage of cells in each phase of the cell cycle.

Principle

Cells stained with the cell cycle dye will fluoresce in proportion to their DNA content.

MultiCyt Cell Cycle Kit
Detection ChanneliQue ScreenerFL3
iQue Screener PLUSBL4
Protocol
  • Prepare and treat cells
  • Stain cells
  • Incubate
  • Read on an iQue Screener
Available SizesCatalog Number
1 x 384 well90261
5 x 384 wells90262
20 x 384 wells90263
50 x 384 wells90264

MultiCyt 4-Plex Apoptosis Kit

Key Benefits

  • No wash protocol – just add and read for fast, easily automated workflow. Our 4-plex kit is easier to run than some single endpoint kits!
  • Four parameters to deliver rich content, including insight on mechanism of action.
  • The 4 components are available separately so you can pick and choose.
  • Multiplex with QBeads PlexScreen kits for richer content.

Kit Description

The 4-Plex Apoptosis Kit is a multi-parameter assay consisting 4 different apoptosis measurements per well (cell viability, caspase 3/7 activity, annexin V binding, and mitochondrial depolarization) for a robust determination of apoptosis progression and insight into mechanism of action. The individual reagent components are also available separately for greater flexibility.

Principle

The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.
Surface expression of phosphatidylserine is detected by the binding of Annexin-V to the cell surface.
Cell viability is determined by the ability of live cells to exclude a DNA binding.
Mitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.

MultiCyt 4-Plex Apoptosis Kit
ReagentsCaspase 3/7 Detection ReagentAnnexin V Binding ReagentCell Viability Detection ReagentMitochondrial Depolarization Detection Reagent
Detection ChanneliQue ScreenerFL1FL2FL3FL4
iQue Screener PLUSBL1BL2BL4RL1
Protocol
  • Prepare and treat cells
  • Stain cells
  • Incubate
  • Read on an iQue Screener
Available SizesCatalog Number
1 x 38490053
5 x 38490054
20 x 38490155
50 x 38490156

MultiCyt Apoptosis Kits (Mix & Match)

Kit Description

Caspase 3/7 Detection Kit consists of a dye that fluoresces when cleaved by activated caspases 3 & 7 which play an important role in the apoptotic pathway. An increase in fluorescence scores as a positive for apoptotic response. This kit can be multiplexed with other MultiCyt kits.

Annexin V Kit detects phosphatidyl serine on the exterior cell membrane. An increase in fluorescence scores as a positive for apoptotic response. This kit can be multiplexed with other MultiCyt kits.

Cell Viability Detection Kit consists of a dye that enables to differentiate live cells from dead cells. An increase in fluorescence scores as a positive for apoptotic response. This kit can be multiplexed with other MultiCyt kits.

Mitochondrial Depolarization Kit consists of a dye that detects mitochondrial depolarization by leaking from the lumen of mitochondria to the cytoplasm. This causes the cell to exhibit decreased fluorescence. This kit can be multiplexed with other MultiCyt kits.

MultiCyt Apoptosis Kits (Mix & Match)
ReagentsCaspase 3/7 Detection ReagentAnnexin V Binding ReagentCell Viability Detection ReagentMitochondrial Depolarization Detection Reagent
Detection ChanneliQue ScreenerFL1FL2FL3FL4
iQue Screener PLUSBL1BL2BL4RL1
Protocol
  • Prepare and treat cells
  • Stain cells
  • Incubate
  • Read on an iQue Screener
Available SizesCatalog Number
5 x 38491034910309034691038
20 x 38491035910319034791039
50 x 38491036910329034891040

MultiCyt Cell Membrane Integrity Kits

Key Benefits

  • Three dye options gives you more ways to measure cell death.
  • No wash protocol, just add and read for fast, easily automated workflow.
  • Multiplex with other MultiCyt kits like Cell Cycle for richer content.

Kit Description

Each Membrane Integrity Kit consists of a spectrally distinct proprietary dye that is taken up by cells with compromised membranes and excluded by healthy cells with intact membranes The Membrane Integrity Kits can be multiplexed with other MultiCyt reagents.

Principle

The Membrane Integrity dyes are fluorescent molecules that are cell impermeant. Healthy cells with intact cell membranes will exclude the dyes. Unhealthy cells with compromised membranes will allow entry of the dye into the intracellular space. Upon entry to the cell, the dye localizes to the nucleus and binds to DNA by intercalation.

MultiCyt Membrane Integrity Kits
Kit TypesFL1 Membrane Integrity KitFL3 Membrane Integrity KitFL4 Membrane Integrity Kit
Detection ChanneliQue ScreenerFL1FL3FL4
iQue Screener PLUSBL1BL4RL1
Protocol
  • Prepare and treat cells
  • Stain cells
  • Incubate
  • Read on an iQue Screener
Available SizesCatalog Number
5 x 384903429034690350
20 x 384903439034790351
50 x 384903449034890352

MultiCyt Cell Encoder Kits

Key Benefits

  • Two dye options gives you more ways to encode cells.
  • No wash protocol, just add and read for fast, easily automated workflow.
  • Multiplex with other MultiCyt kits like Cell Membrane Integrity for richer content.
  • Dyes are safe and non-toxic to you and the cells!

Kit Description

The MultiCyt Cell Encoder Kits enable labeling of many standard cell lines by using different intensities of a single fluorophore. Each kit includes one dye on a single fluorescent channel that are titrated to three different concentrations to label cells at none, dim, mid, and bright intensity.

Principle

Fluorescent cell encoding is a technique where different cell populations are labeled with different intensities of a fluorophore, establishing a unique signature of fluorescence intensity that enables the ability to analyze multiple cell lines simultaneously.

MultiCyt Cell Encoder Kits
TypesMultiCyt FL1 Cell Encoder KitMultiCyt FL4 Cell Encoder Kit
Detection ChanneliQue ScreenerFL1FL4
iQue Screener PLUSBL1RL1
Protocol
  • Add dye to cells at different concentrations
  • Incubate cells
  • Wash cells
  • Plate cells
  • Read on the iQue Screener
Available SizesCatalog Number
5x 384 wells9035490358
20x 384 wells9035590359
50x 384 wells9035690360

MultiCyt Cell Proliferation Kits

Key Benefits

  • Two dye options gives you more ways to measure cell proliferation.
  • No wash protocol, just add and read for fast, easily automated workflow.
  • Multiplex with other MultiCyt kits like QBeads PlexScreen kits for richer content.
  • Dyes are safe and non-toxic to you and the cells!

Kit Description

Each Cell Proliferation Kit consists of a spectrally distinct proprietary fluorescent dye that is cell permeable.
The Cell Proliferation Kits can be multiplexed with other MultiCyt reagents.

Principle

The Cell Proliferation Kits function on the principle of dye dilution. Cell proliferation is detected and quantified based on the halving or “dilution” of the loaded dye after each round of cellular division.

MultiCyt Cell Proliferation Kits
Kit TypesFL1 Cell Proliferation KitFL4 Cell Proliferation Kit
Detection ChanneliQue ScreenerFL1FL4
iQue Screener PLUSBL1RL1
Protocol
  • Prepare cells
  • Stain cells
  • Incubate
  • Read on the iQue Screener
Available SizesCatalog Number
5 X 384 wells9035490358
20 X 384 wells9035590359
50 X 384 wells9035690360

Genotoxicity/Micronucleus Kit

Key Benefits

  • Analyze hundreds of samples in minutes!
  • Analyze suspension or adherent cell lines with flow.
  • Distinguish between Aneugens and Clastogens in the same assay.
  • Includes cell viability readout for richer content.

Kit Description

The Genotoxicity/Micronucleus Kit has two dye components. One dye indicates cellular toxicity, the second dye indicates nuclei size. These are added sequentially to differentially stain nuclei from healthy vs membrane compromised cells. Fluorescent marker beads are included so that specific counts of the nuclei can be made .The number and size of stained nuclei are then analyzed.

Principle

The Genotoxicity/Micronucleus Kit has two dye components that are added sequentially to differentially stain nuclei from healthy vs membrane compromised cells. The number and size of stained nuclei are then analyzed.

Genotoxicity/Micronucleus Kit
Detection ChanneliQue ScreenerFL1FL4
iQue Screener PLUSBL1RL1
Protocol
  • Seed cells
  • Treat cells
  • Stain cells
  • Lyse cells; stain nuclei
  • Read on the iQue Screener
Available SizeCatalog Number
5 X 384 wells90153

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