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Intellicyt® Antibody Internalization Reagent

February 27, 2019

Key Benefits

  • No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization
  • High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 20 µL of cells and antibody.
  • Cell viability can also be measured by multiplexing Intellicyt® Cell Membrane Integrity Dye to assess general cell health and antibody function.
  • Cell specificity can be characterized by multiplexing Intellicyt® Encoding dyes (cell lines) or directly conjugated fluorescent antibodies (cells).
  • The QBeads® Assay can also be multiplexed to perform more detailed antibody assessments on the sample, such as cytokine release.
  • Available in Human and Mouse formats

Kit Description

The Intellicyt Antibody Internalization is designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 20 µL of cells and antibody.

Principle

Antibodies are easily labeled with Intellicyt Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.

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Product NameAvailable SizesCatalog No
Intellicyt® Antibody Internalization Human Reagent1 x 9690564
1 x 38490565
Intellicyt® Antibody Internalization Mouse Reagent1 x 9690566
1 x 38490567

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