Visit booth #202 to learn the latest on the Intellicyt® iQue Screener PLUS
- Multiplexed binding assays (96-well plate in 5 min)
- Identify targets in their native conformation
- Evaluate specificity and species cross-reactivity in the same well
- Get information on cell health, isotype and titer from a single assay
Attend our poster presentation to learn how our high throughput suspension cell and bead analysis platform helps accelerate discovery and development of novel therapeutics and provides new insight into the mechanisms of disease at the speed, depth and scale not achievable with conventional techniques.
Development of a High-Throughput Multiplex Mouse IgG Isotyping and Quantitation Assay
Presenter: Carrie Weldon, Senior Research Associate
We describe a high-throughput, multiplexed, no wash assay that measures antibody internalization, antibody specificity, and cell health from a single, 10uL sample. This assay is amenable to profiling large number of antibodies in 96- and 384-well plate formats.
Antibodies are easily labeled with the novel, pH sensitive dye (Intellicyt® Antibody Internalization Reagent R/Red). The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they were internalized and processed through the acidic lysosome/endosome pathway.
Cell viability can also be measured using MultiCyt® Cell Membrane Integrity Dye (Intellicyt) to assess general cell health and antibody function. Using the same sample, cell specificity can be characterized using encoding dyes (cell lines) or directly conjugated fluorescent antibodies (cells).
The Qbeads® Assay (Intellicyt) can also be incorporated to perform more detailed antibody assessments on the sample, such as cytokine release.
Data acquisition can be efficiently captured using the Intellicyt® iQue Screener PLUS platform, which combines high throughput sampling, flow cytometry detection, and on board analysis with plate level analytics that deliver rich content with sampling times of less than 20 minutes for a 384-well plate. The integrated ForeCyt® software enables data analysis and visualization.