Resources, User Publications

Complete List of User Publications

Year: 2018

High-Throughput Flow Cytometric Method for the Simultaneous Measurement of CAR-T Cell Characterization and Cytotoxicity against Solid Tumor Cell Lines

Martinez, E. M., Klebanoff, S. D., Secrest, S., Romain, G., Haile, S. T., Emtage, P. C., & Gilbert, A. E. (2018). High-Throughput Flow Cytometric Method for the Simultaneous Measurement of CAR-T Cell Characterization and Cytotoxicity against Solid Tumor Cell Lines. SLAS DISCOVERY: Advancing Life Sciences R&D, 23(7), 603-612. doi:10.1177/2472555218768745

 

Automated High-Throughput Flow Cytometry for High-Content Screening in Antibody Development

Wang, Y., Yoshihara, T., King, S., Le, T., Leroy, P., Zhao, X., . . . Menon, S. (2018, June 13). Automated High-Throughput Flow Cytometry for High-Content Screening in Antibody Development. Retrieved from

 

Application of High-Throughput Flow Cytometry in Early Drug Discovery: An AstraZeneca Perspective

Ding, M., Clark, R., Bardelle, C., Backmark, A., Norris, T., Williams, W., .  Howes, R. (2018). Application of High-Throughput Flow Cytometry in Early Drug Discovery: An AstraZeneca Perspective. SLAS DISCOVERY: Advancing Life Sciences R&D, 247255521877507. doi:10.1177/2472555218775074

 

Emerging technologies for biotherapeutic bioanalysis from a high-throughput and multiplexing perspective: insights from an AAPS emerging technology action program committee

Purushothama, S., Dysinger, M., Chen, Y., Österlund, K., Mora, J., Chunyk, A. G., & Peloquin, R. (2018). Emerging technologies for biotherapeutic bioanalysis from a high-throughput and multiplexing perspective: Insights from an AAPS emerging technology action program committee. Bioanalysis, 10(3), 181-194. doi:10.4155/bio-2017-0196

 

A Multiplexed Screening Assay to Evaluate Chemotherapy-Induced Myelosuppression Using Healthy Peripheral Blood and Bone Marrow

Javarappa, K. K., Tsallos, D., & Heckman, C. A. (2018). A Multiplexed Screening Assay to Evaluate Chemotherapy-Induced Myelosuppression Using Healthy Peripheral Blood and Bone Marrow. SLAS DISCOVERY: Advancing Life Sciences R&D, 247255521877796. doi:10.1177/2472555218777968

 

Comparing Flow Cytometry QBeads PlexScreen Assays with Other Immunoassays for Determining Multiple Analytes

Ding, M., Cavallin, A., Hermansson, N., Berntsson, P., Jinton, L., & Blomqvist, S. R. (2018). Comparing Flow Cytometry QBeads PlexScreen Assays with Other Immunoassays for Determining Multiple Analytes. SLAS DISCOVERY: Advancing Life Sciences R&D, 247255521877161. doi:10.1177/2472555218771610

 

New Methods Improve Ab Engineering

Visk, D. (2018). New Methods Improve Ab Engineering. Genetic Engineering & Biotechnology News, 38(4), 10-11. doi:10.1089/gen.38.04.05

 

Rational Design and Development of a Peptide Inhibitor for the PD-1/PD-L1 INTERACTION

Boohaker, R. J., Sambandam, V., Segura, I., Miller, J., Suto, M., & Xu, B. (2018). Rational Design And Development Of A Peptide Inhibitor For The Pd-1/pd-L1 Interaction. Cancer Letters. doi:10.1016/j.canlet.2018.04.031

 

Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis

Angelini, A., Miyabe, Y., Newsted, D., Kwan, B. H., Miyabe, C., Kelly, R. L., . . . Wittrup, K. D. (2018). Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis. Nature Communications, 9(1). doi:10.1038/s41467-018-03687-x

 

Techniques to Enable 1536-Well Phenotypic Screening

Knight, S., Plant, H., Mcwilliams, L., & Wigglesworth, M. (2018). Techniques to Enable 1536-Well Phenotypic Screening. Methods in Molecular Biology Phenotypic Screening, 263-278. doi:10.1007/978-1-4939-7847-2_20

 

Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries

Kelly, R. L., Le, D., Zhao, J., & Wittrup, K. D. (2018). Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries. Journal of Molecular Biology, 430(1), 119-130. doi:10.1016/j.jmb.2017.11.008

 

Defective immuno-and thymoproteasome assembly causes severe immunodeficiency

Treise, I., Huber, E. M., Klein-Rodewald, T., Heinemeyer, W., Grassmann, S. A., Basler, M., . . . Busch, D. H. (2018). Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. Scientific Reports, 8(1). doi:10.1038/s41598-018-24199-0

 

Aggressive natural killer-cell leukemia mutational landscape and drug profiling highlight JAK-STAT signaling as therapeutic target

Dufva, O., Kankainen, M., Kelkka, T., Sekiguchi, N., Awad, S. A., Eldfors, S., . . . Mustjoki, S. (2018). Aggressive natural killer-cell leukemia mutational landscape and drug profiling highlight JAK-STAT signaling as therapeutic target. Nature Communications, 9(1). doi:10.1038/s41467-018-03987-2

 

DNA-based biomimetics as modular tools to study reconstituted and cellular systems

L., & Sophie, J. (2018, January 17). DNA-based biomimetics as modular tools to study reconstituted and cellular systems. Retrieved from

 

High-throughput flow cytometric method for the simultaneous measurement of CAR-T cell characterization and cytotoxicity against solid tumor cell lines.

Martinez, E., Klebanoff, S., Secrest, S., Romain, G., Haile, S., Emtage, P, Gilbert, A. (2018), High-throughput flow cytometric method for the simultaneous measurement of CAR-T cell characterization and cytotoxicity against solid tumor cell lines. SLAS Discovery. doi:10.1177/2472555218768745

 

 

Year: 2017

Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins. Nucleic Acids Research

Yang, N. J., Kauke, M. J., Sun, F., Yang, L. F., Maass, K. F., Traxlmayr, M. W., . . . Wittrup, K. D. (2017). Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins. Nucleic Acids Research, 45(13), 7602-7614. doi:10.1093/nar/gkx546

 

Polyfunctional response by ImmTAC (IMCgp100) redirected CD8+ and CD4+ T cells

Boudousquie, C., Bossi, G., Hurst, J. M., Rygiel, K. A., Jakobsen, B. K. and Hassan, N. J. (2017), Polyfunctional response by ImmTAC (IMCgp100) redirected CD8+ and CD4+ T cells. Immunology. doi:10.1111/imm.12779

 

High throughput combinatorial profiling of checkpoint inhibitor antibodies on the iQue® Screener PLUS

Hasaka, T. (2017). Abstract 5594: High throughput combinatorial profiling of checkpoint inhibitor antibodies on the iQue® Screener PLUS. Cancer Research, 77(13 Supplement), 5594-5594. doi:10.1158/1538-7445.am2017-

 

An engineered protein antagonist of K-Ras/B-Raf interaction

Kauke, M. J., Traxlmayr, M. W., Parker, J. A., Kiefer, J. D., Knihtila, R., McGee, J., … Wittrup, K. D. (2017). An engineered protein antagonist of K-Ras/B-Raf interaction. Scientific Reports, 7, 5831. http://doi.org/10.1038/s41598-017-05889-7

 

Candidiasis and the impact of flow cytometry on antifungal drug discovery

Ku, T. S., Bernardo, S., Walraven, C. J., & Lee, S. A. (2017). Candidiasis and the impact of flow cytometry on antifungal drug discovery. Expert Opinion on Drug Discovery, 1-11. doi:10.1080/17460441.2017.1377179

 

High-throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers

Sullivan, J. T., Sulli, C., Nilo, A., Yasmeen, A., Ozorowski, G., Sanders, R. W., . . . Doranz, B. J. (2017). High-throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers. Journal of Virology. doi:10.1128/jvi.00862-17

 

High-throughput flow cytometry for drug discovery: principles, applications, and case studies. Drug Discovery Today

Ding, M., Kaspersson, K., Murray, D., & Bardelle, C. (2017). High-throughput flow cytometry for drug discovery: principles, applications, and case studies. Drug Discovery Today. doi:10.1016/j.drudis.2017.09.005

 

Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors

Kyoizumi, S., Kubo, Y., Kajimura, J., Yoshida, K., Hayashi, T., Nakachi, K., . . . Kusunoki, Y. (2017). Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors. The Journal of Immunology. doi:10.4049/jimmunol.1601711

 

Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody

Kenniston, J. A., Taylor, B. M., Conley, G. P., Cosic, J., Kopacz, K. J., Lindberg, A. P., . . . Nixon, A. E. (2017). Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody. Journal of Biological Chemistry. doi:10.1074/jbc.m117.807396

 

The Short Isoform of BRD4 Promotes HIV-1 Latency by Engaging Repressive SWI/SNF Chromatin-Remodeling Complexes

Conrad, R. J., Fozouni, P., Thomas, S., Sy, H., Zhang, Q., Zhou, M., & Ott, M. (2017). The Short Isoform of BRD4 Promotes HIV-1 Latency by Engaging Repressive SWI/SNF Chromatin-Remodeling Complexes. Molecular Cell, 67(6). doi:10.1016/j.molcel.2017.07.025

 

High-throughput screening of hybridoma supernatants using multiplexed fluorescent cell barcoding on live cells

Lu, M., Chan, B. M., Schow, P. W., Chang, W. S., & King, C. T. (2017). High-throughput screening of hybridoma supernatants using multiplexed fluorescent cell barcoding on live cells. Journal of Immunological Methods. doi:10.1016/j.jim.2017.08.002

 

A Novel Clone Ranking and Selection Assay that Combines all the Relevant Insights Needed to Choose the Most Optimal Clones in a Single Experiment

Duensing, Z. Liu, and C. Hauser (2017). A Novel Clone Ranking and Selection Assay that Combines all the Relevant Insights Needed to Choose the Most Optimal Clones in a Single Experiment, J Immuno May 1, 2017,         (1 Supplement) 213.4

 

 

Year: 2016

Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of VL and VH Domains Targeting CD123+ Tumors

Thokala, S. Olivares, T. Mi, S. Maiti, D. Deniger, H. Huls, H. Torikai, H. Singh, R. E. Champlin, T. Laskowski, G. McNamara, L. J. N. Cooper (2016). Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of VL and VH Domains Targeting CD123+ Tumors, http://dx.doi.org/10.1371/journal.pone.0159477

 

Human Antibodies that Recognize Novel Immunodominant Quaternary Epitopes on the HIV-1 Env Protein

D. Hicar, X. Chen, C. Sulli, T. Barnes, J. Goodman, H. Sojar, B. Briney, J. Willis, V. U. Chukwuma, S. A. Kalams, B. J. Doranz, P. Spearman, J. E. Crowe Jr. (2016). Human Antibodies that Recognize Novel Immunodominant Quaternary Epitopes on the HIV-1 Env Protein, http://dx.doi.org/10.1371/journal.pone.0158861

 

Development of a Human Whole Blood Screening Platform to Monitor JAK/STAT Signaling Using High-Throughput Flow Cytometry

P. Fereshteh, X. L. Sha Li, Y. Fan, R. Zhang, G. A. Farr, Kolodin, J. Lippy, J. G. Naglich, G. Schieven, L. Schweizer, L. Zhang. (2016). Development of a Human Whole Blood Screening Platform to Monitor JAK/STAT Signaling Using High-Throughput Flow Cytometry, Bristol-Myers Squibb Company, Princeton, NJ, USA, Sanofi-Aventis, Boston, MA, USA; J Biomol Screen, pii: 1087057116645095

 

 

Year: 2015

A High-Throughput Phenotypic Screen of Cytotoxic T Lymphocyte Lytic Granule Exocytosis Reveals Candidate Immunosuppressant

Zhao, M. K. Haynes, O. Ursu, B. S. Edwards, L. A. Sklar, and A. Zweifach. (2015). A High-Throughput Phenotypic Screen of Cytotoxic T Lymphocyte Lytic Granule Exocytosis Reveals Candidate Immunosuppressants, J Biomol Screen, doi: 20: 359 – 371.

 

AGS67E, an Anti-CD37 Monomethyl Auristatin E Antibody–Drug Conjugate as a Potential Therapeutic for B/T-Cell Malignancies and AML: A New Role for CD37 in AML

Pereira, C. I. Guevara, L. Jin, N. Mbong, A. Verlinsky, S. J. Hsu, H. Aviña, S. Karki, J. D. Abad, P. Yang, S. Moon, F. Malik, M. Y. Choi, Z. An, K. Morrison, P. M. Challita-Eid, F. Doñate, I. B.J. Joseph, T. J. Kipps, J. E. Dick, and D.R. Stover. (2015). AGS67E, an Anti-CD37 Monomethyl Auristatin E Antibody–Drug Conjugate as a Potential Therapeutic for B/T-Cell Malignancies and AML: A New Role for CD37 in AML, Mol. Cancer Ther., 14: 1650 – 1660.

 

A Cell-Based Internalization and Degradation Assay with an Activatable Fluorescence–Quencher Probe as a Tool for Functional Antibody Screening

Li, P. C. Liu, Y. Shen, M. D. Snavely, K. Hiraga, J. Biomol Screen, (2015). A Cell-Based Internalization and Degradation Assay with an Activatable Fluorescence–Quencher Probe as a Tool for Functional Antibody Screening, doi:10.1177/1087057115588511.

 

Condition-specific genetic interaction maps reveal crosstalk between the cAMP/PKA and the HOGMAPK pathways in the activation of the general stress respons

Gutin1, A. Sadeh1, A. Rahat1, A. Aharoni2, N. Friedman1. (2015). Condition-specific genetic interaction maps reveal crosstalk between the cAMP/PKA and the HOG MAPK pathways in the activation of the general stress response, Mol Syst Biol, 11: 829.

 

Profiling a structure activity relationship library for immunological activity in human PBMCs: multiplexed measurements of cell phenotype, viability, and cytokine secretion from a single high throughput screen (TECH2P.923)

Duensing, Z. Liu, D. Brodbeck, S. Parel, and Y. L. J. Immunol., (2015). Profiling a structure activity relationship library for immunological activity in human PBMCs: multiplexed measurements of cell phenotype, viability, and cytokine secretion from a single high throughput screen (TECH2P.923), 194: 206.33.

 

Identification of Novel Radiosensitizers in a High-Throughput, Cell-Based Screen for DSB Repair Inhibitor

G. Goglia, R. Delsite, A. N. Luz, D. Shahbazian, A. F. Salem, R. K. Sundaram, J. Chiaravalli, P. J. Hendrikx, J. A. Wilshire, M. Jasin, H. M. Kluger, J. F. Glickman, S. N. Powell, and R. S. Bindra. (2015). Identification of Novel Radiosensitizers in a High-Throughput, Cell-Based Screen for DSB Repair Inhibitors, Mol. Cancer Ther., 14: 326 – 342.

 

 

Year: 2014

HIV-1 Infection and Antibodies to Plasmodium falciparum in Adults

Hasang, E. G. Dembo, R.Wijesinghe, M. E. Molyneux, J. G. Kublin, and S.Rogerson (2014). HIV-1 Infection and Antibodies to Plasmodium falciparum in Adults. The Journal of Infectious Disease, 210: 1407 – 1414.

 

Exposure of Epitope Residues on the Outer Face of the Chikungunya Virus Envelope Trimer Determines Antibody Neutralizing Efficacy

H. Fong, S. S. R. Banik, K. Mattia, T. Barnes, D.Tucker, N. Liss, K. Lu, S. Selvarajah, S. Srinivasan, M. Mabila, A. Miller, M. O. Muench, A. Michault, J. B. Rucker, C. Paes, G. Simmons, K. M. Kahle, and B.J. Doranz, J. Virol., (2014). Exposure of Epitope Residues on the Outer Face of the Chikungunya Virus Envelope Trimer Determines Antibody Neutralizing Efficacy, 88: 14364 – 14379.

 

Mapping Small Effect Mutations in Saccharomyces cerevisiae: Impacts of Experimental Design and Mutational Properties

Duveau, Brian P. H. Metzger, J. D. Gruber, K. Mack, N. Sood, T. E. Brooks, and P. J. Wittkop. (2014). Mapping Small Effect Mutations in Saccharomyces cerevisiae: Impacts of Experimental Design and Mutational Properties, Pg3; 4: 1205 – 1216.

 

GEN Tutorial: Phenotypic Compound Profiling: Applying Multiplexing Capabilities to a Range of Drug Discovery Activities

Luu, D.  Brodbeck. (2014). Genetic Engineering News Discovery Activities, (Vol. 34, No. 2), Developed to complement high-content imaging, the iQue Screener is optimized for suspension assay formats with cells, beads, microbes, and mixtures. The platform provides a mechanism for subpopulation phenotyping—where multiple subpopulations are identified by size and density then correlated with intensities of multiple fluorescent probes on a per object basis. Researchers can rapidly profile compound effects on nonadherent cell lines, including primary immune system cells, and determine molecular interactions using cell or bead-based assays. Read on at the Genetic Engineering News website.

 

Low Antibody Levels to Pregnancy-specific Malaria Antigens and Heightened Cytokine Responses Associated With Severe Malaria in Pregnancy

P. Chandrasiri, L. M. Randall, A. A. Saad, A. M. Bashir, S. J. Rogerson, and I. Adam. (2014) The Journal of Infectious Disease, Low Antibody Levels to Pregnancy-specific Malaria Antigens and Heightened Cytokine Responses Associated With Severe Malaria in Pregnancy, 209: 1408 – 1417.

 

Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity

B. Messer, R. de Alwis, B. L. Yount, S. R. Royal, J. P. Huynh, S. A. Smith, J. E. Crowe, Jr., B. J. Doranz, K. M. Kahle, J. M. Pfaff, L. J. White, C. A. Sariol, A. M. de Silva, R. S. Baric, PNAS. (2014). Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity,111: 1939 – 1944.

 

Origin and Differential Selection of Allelic Variation at TAS2R16 Associated with Salicin Bitter Taste Sensitivity in Africa

M.C. Campbell, A. Ranciaro, D. Zinshteyn, R. Rawlings-Goss, J. Hirbo, S. Thompson, D. Woldemeskel, A. Froment, J. B. Rucker, S. A. Omar, J. Bodo, T. Nyambo, G. Belay, D. Drayna, P. A. S. Breslin, and S. A. Tishkoff. Mol. Biol. Evol. (2014). Origin and Differential Selection of Allelic Variation at TAS2R16 Associated with Salicin Bitter Taste Sensitivity in Africa, 31: 288 – 302.

 

Antibodies: A Laboratory Manual Second Edition.Antibody Screening using High Throughput Flow Cytometry

Duensing, S. Watson. (2014). Antibodies: A Laboratory Manual Second Edition. Antibody Screening using High Throughput Flow Cytometry, Cold Spring Harbor Laboratory Press.

 

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors

Gispert, D. Parganlija, M. Klinkenberg, S. Dröse, I. Wittig, M. Mittelbronn, P. Grzmil, S. Koob, A. Hamann, M. Walter, F. Büchel, T. Adler, M. Hrabé de Angelis, D. H. Busch, A. Zell, A. S. Reichert, U. Brandt, H. D. Osiewacz, M. Jendrach, and G. Auburger.(2013). Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors, Hum. Mol. Genet., 22: 4871 – 4887.

 

Atomic-level functional model of dengue virus Envelope protein infectivity

A. Christian, K. M. Kahle, K. Mattia, B. A. Puffer, J. M. Pfaff, A. Miller, C. Paes, E. Davidson, and B. J. Doranz. (2013). Atomic-level functional model of dengue virus Envelope protein infectivity, PNAS, 110: 18662 – 18667.

 

Functional Analysis of Antibodies against Dengue Virus Type 4 Reveals Strain-Dependent Epitope Exposure That Impacts Neutralization and Protection

Sukupolvi-Petty, J. D. Brien, S. K. Austin, B. Shrestha, S. Swayne, K. Kahle, B. J. Doranz, S. Johnson, T. C. Pierson, D. H. Fremont, and M. S. Diamond. J. Virol. (2013). Functional Analysis of Antibodies against Dengue Virus Type 4 Reveals Strain-Dependent Epitope Exposure That Impacts Neutralization and Protection, 87: 8826 – 8842.

 

 

Year: 2013

High-Content Screening: Imaging, Analysis, and Applications

Mattheakis, J. Biomol Screen. (2013). High-Content Screening: Imaging, Analysis, and Applications, 18: 845 – 847.

 

High-Throughput Secondary Screening at the Single-Cell Level

P. Robinson, V. Patsekin, C. Holdman, K. Ragheb, J. Sturgis, R. Fatig, L. V. Avramova, B. Rajwa, V.  Davisson, N. Lewis, P. Narayanan, N.  Li, and C. W. Qualls. (2013). High-Throughput Secondary Screening at the Single-Cell Level, Jr. Journal of Laboratory Automation, 18: 85 – 98.

 

Flow Cytometry Enables a High-Throughput Homogeneous Fluorescent Antibody-Binding Assay for Cytotoxic T Cell Lytic Granule Exocytosis

E. Florian, C. K. Lepensky, O. Kwon, M. K. Haynes, L. A. Sklar, and A. Zweifach. (2013). Flow Cytometry Enables a High-Throughput Homogeneous Fluorescent Antibody-Binding Assay for Cytotoxic T Cell Lytic Granule Exocytosis, J Biomol Screen, 18: 420 – 429.

 

Selective Agonists and Antagonists of Formylpeptide Receptors: Duplex Flow Cytometry and Mixture-Based Positional Scanning Libraries

Pinilla, B. S. Edwards, J. R. Appel, T. Yates-Gibbins, M. A. Giulianotti, J. L. Medina-Franco, S. M. Young, R. G. Santos, L. A. Sklar, and R. A. Houghten. (2013). Selective Agonists and Antagonists of Formylpeptide Receptors: Duplex Flow Cytometry and Mixture-Based Positional Scanning Libraries, Mol. Pharmacol., 84: 314 – 324.

 

Characterization of a Cdc42 Protein Inhibitor and Its Use as a Molecular Probe

Hong, S. R. Kenney, Genevieve K. Phillips, D. Simpson, C. E. Schroeder, J. Nöth, E. Romero, S. Swanson, A. Waller, J. J. Strouse, M. Carter, A. Chigaev, O. Ursu, T. Oprea, B. Hjelle, J. E. Golden, J. Aubé, L. G. Hudson, T. Buranda, L. A. Sklar, and A. Wandinger-Ness. J. Biol. Chem. (2013). Characterization of a Cdc42 Protein Inhibitor and Its Use as a Molecular Probe, 288: 8531 – 8543.

 

A Selective ATP-Binding Cassette Subfamily G Member 2 Efflux Inhibitor Revealed via High-Throughput Flow Cytometry

Jacob Strouse, I. Ivnitski-Steele, H. M. Khawaja, D. Perez, J. Ricci, T. Yao, W. S. Weiner, C. E. Schroeder, D. S. Simpson, B. E. Maki, K. Li, J. E. Golden, T. D. Foutz, A. Waller, A. M. Evangelisti, S. M. Young, S. E. Chavez, M. J. Garcia, O. Ursu, C. G. Bologa, M. B. Carter, V. M. Salas, K. Gouveia, G. P. Tegos, T. I. Oprea, B. S. Edwards, J. Aubé, R. S. Larson, and L. A. Sklar. (2013). A Selective ATP-Binding Cassette Subfamily G Member 2 Efflux Inhibitor Revealed via High-Throughput Flow Cytometry, J Biomol Screen, 18: 26 – 38.

 

Multiplexing high-content flow (HCF) and quantitative high-throughput screening (qHTS) to identify compounds capable of decreasing cell viability, activating caspase 3/7, expressing annexin V, and changing mitochondrial membrane integrity.

LA, K. JM, C. McKnight, S. Michael, P. Shinn, D. Liu, LM Staudt, CJ Thomas, M. Ferrer. (2013). Multiplexing high-content flow (HCF) and quantitative high-throughput screening (qHTS) to identify compounds capable of decreasing cell viability, activating caspase 3/7, expressing annexin V, and changing mitochondrial membrane integrity, Curr Protoc Chem Biol, 1;5(3):195-212.

 

Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells

A. Quiñones, T. I. Moore, K. Nicholes, H. Lee, S. Kim, L. Sun, N. Li Jeon, and J. Stephan. (2013). Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells, Blood, 121: e25 – e33.

 

Evolution of Biologics Screening Technologies

Cariuk et.al. (2013). Evolution of Biologics Screening Technologies, Review article on antibody discovery developments, including Intellicyt technology, Pharmaceuticals, 6, 681-688; doi: 10.3390/ph6050681.

 

Dissection of the BCR-ABL signaling network using highly specific monobody inhibitors to the SHP2 SH2 domains

Sha, E. B. Gencer, S. Georgeon, A. Koide, N. Yasui, S. Koide, and O. Hantschel PNAS, (2013).  Dissection of the BCR-ABL signaling network using highly specific monobody inhibitors to the SHP2 SH2 domains, 110: 14924 – 14929.

 

High-Throughput Secondary Screening at the Single-Cell Level

P. Robinson, V. Patsekin, C. Holdman, K. Ragheb, J. Sturgis, R.  Fatig, L. V. Avramova, B. Rajwa, V. Jo Davisson, N. Lewis, P. Narayanan, N. Li, and C. W. Qualls, Jr. (2013) High-Throughput Secondary Screening at the Single-Cell Level, Journal of Laboratory Automation, 18: 85 – 98.

 

The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein

A. Smith, A. Ruklanthi de Alwis, N. Kose, E. Harris, K. D. Ibarra, K. M. Kahle, J.M. Pfaff, X. Xiang, B. J. Doranz, A. M. de Silva, S. K. Austin, S. Sukupolvi-Petty, M. S. Diamond, and J. E. Crowe. (2013). The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein, Jr. mBio, 4: e00873-13.

 

 

Year: 2012

Selected Drugs with Reported Secondary Cell-Differentiating Capacity Prime Latent HIV-1 Infection for Reactivation

Shishido, F. Wolschendorf, A. Duverger, F. Wagner, J. Kappes, J. Jones, O. Kutsch, J. Virol. (2012). Selected Drugs with Reported Secondary Cell-Differentiating Capacity Prime Latent HIV-1 Infection for Reactivation, 86: 9055 – 9069.

 

New tools for classification and monitoring of autoimmune disease

T. Maecker, T. M. Lindstrom, W. H. Robinson, P. J. Utz, M. Hale, S. D. Boyd, S. S. Shen-Orr, and C. G. Fathman. (2012). New tools for classification and monitoring of autoimmune diseases, Nat Rev Rheumatol., 8(6): 317–328.

 

Profiling of Toxicity and Identification of Distinct Apoptosis Profiles Using a 384-Well High-Throughput Flow Cytometry Screening Platform

Y.K. Luu, P. l. Rana, T. D. Duensing, C. Black, Y. Will. (2012). Profiling of Toxicity and Identification of Distinct Apoptosis Profiles Using a 384-Well High-Throughput Flow Cytometry Screening Platform, J Biomol Screen, 17: 806 – 812.

 

Phaedra, a Protocol-Driven System for Analysis and Validation of High-Content Imaging and Flow Cytometry

Cornelissen, M. Cik, and E. Gustin. (2012). Phaedra, a Protocol-Driven System for Analysis and Validation of High-Content Imaging and Flow Cytometry, J Biomol Screen, 17: 496 – 506.

 

Discovery of Regulators of Receptor Internalization with High-Throughput Flow Cytometry

Wu, P. H. Tapia, G. W. Fisher, P. C. Simons, J. J. Strouse, T. Foutz, A. S. Waggoner, Jonathan Jarvik, and Larry A. Sklar. (2012). Discovery of Regulators of Receptor Internalization with High-Throughput Flow Cytometry, Mol. Pharmacol, 82: 645 – 657.

 

Testing the Oxidative Stress Hypothesis of Aging in Primate Fibroblasts: Is There a Correlation Between Species Longevity and Cellular ROS Production?

A.Csiszar, A. Podlutsky, N. Podlutskaya, W. E. Sonntag, S. Z. Merlin, E. E. R. Philipp, K. Doyle, A. Davila, F. A. Recchia, P. Ballabh, J. T. Pinto, and Z. Ungvari.(2012).Testing the Oxidative Stress Hypothesis of Aging in Primate Fibroblasts: Is There a Correlation Between Species Longevity and Cellular ROS Production?, J Gerontol, A Biol Sci Med Sci ,67: 841 – 852.

 

Identifying Small Molecules That Overcome HoxA9-Mediated Differentiation Arrest in Acute Myeloid Leukemia

B. Sykes, M. K Haynes, N. Tolliday, A. Waller, J. M. Cobert, B. A. Szekely, T. P. Hasaka, L. Sklar, L. I. Zon, and D. T. Scadden. (2012). Identifying Small Molecules That Overcome HoxA9-Mediated Differentiation Arrest in Acute Myeloid Leukemia, Blood (ASH Annual Meeting Abstracts), 120: 3513.

 

Inhibitors of V-ATPase Proton Transport Reveal Uncoupling Functions of Tether Linking Cytosolic and Membrane Domains of V0 Subunit a (Vph1p)

Chan, C. Prudom, S. M. Raines, S. Charkhzarrin, S. D. Melman, L. P. De Haro, C. Allen, S. A. Lee, L. A. Sklar, and K. J. Parra. (2012). Inhibitors of V-ATPase Proton Transport Reveal Uncoupling Functions of Tether Linking Cytosolic and Membrane Domains of V0 Subunit a (Vph1p), J. Biol. Chem., 287: 10236 – 10250.

 

The Monoamine Oxidase A Inhibitor Clorgyline Is a Broad-Spectrum Inhibitor of Fungal ABC and MFS Transporter Efflux Pump Activities Which Reverses the Azole Resistance of Candida albicans and Candida glabrata Clinical Isolates

Holmes, Mikhail V. Keniya, Irena Ivnitski-Steele, Brian C. Monk, Erwin Lamping, Larry A. Sklar, and Richard D. Cannon. Antimicrob. (2012). The Monoamine Oxidase A Inhibitor Clorgyline Is a Broad-Spectrum Inhibitor of Fungal ABC and MFS Transporter Efflux Pump Activities Which Reverses the Azole Resistance of Candida albicans and Candida glabrata Clinical Isolates, Agents Chemother, 56: 1508 – 1515.

 

 

Year: 2011

In vitro and in vivo activity of frenolicin B against Plasmodium falciparum and P berghei

T. Fitzgerald 1,6, P. P. Henrich2,6, C. O’Brien 2, M. Krause3, E. H. Ekland2, C. Mattheis2, J. M Sa´3, D. Fidock2,4 and C. Khosla1,5. In vitro and in vivo activity of frenolicin B against Plasmodium falciparum and P berghei, The Journal of Antibiotics advance online publication, (2011); doi:10.1038/ja.2011.94.

 

The proteomics of quiescent and nonquiescent cell differentiation in yeast stationary-phase cultures

S. Davidson, R. M. Joe, S. Roy, O. Meirelles, C. P. Allen, M. R. Wilson, P. H. Tapia, E. E. Manzanilla, A. E. Dodson, S. Chakraborty, M. Carter, S. Young, B. Edwards, L. Sklar, and M. Werner-Washburne. (2011). The proteomics of quiescent and nonquiescent cell differentiation in yeast stationary-phase cultures, Mol. Biol. Cell, 22: 988 – 998.

 

Discovery of Very Late Antigen-4 (VLA-4, {alpha}4_1 Integrin) Allosteric Antagonists

Alexandre Chigaev, Yang Wu, D. Bart Williams, Yelena Smagley, and Larry A. Sklar. (2011). Discovery of Very Late Antigen-4 (VLA-4, {alpha}4_1 Integrin) Allosteric Antagonists, J. Biol. Chem., 286: 5455 – 5463.

 

 

Year: 2010

Identification of a Small GTPase Inhibitor Using a High-Throughput Flow Cytometry Bead-Based Multiplex Assay

Surviladze, A. Waller, Y. Wu, E. Romero, B. S. Edwards, A. Wandinger-Ness, and L. A. Sklar. (2010). Identification of a Small GTPase Inhibitor Using a High-Throughput Flow Cytometry Bead-Based Multiplex Assay, J Biomol Screen, 15: 10 – 20.

 

High throughput flow cytometry for discovery at UNMCMD and the NIH Molecular Libraries Initiative

A. Sklar, B. S. Edwards. (2010). High throughput flow cytometry for discovery at UNMCMD and the NIH Molecular Libraries Initiative, Drug Discovery World, How Intellicyt’s technology has been adopted in a compound screening environment. Application areas reviewed include transporters, GPCRs and bead-based ligand-ligand and protein binding assays.

 

 

Year: 2009

Flow Cytometry: A Multipurpose Technology for a Wide Spectrum of Global Biosecurity Applications

L. Marrone. (2009). Flow Cytometry: A Multipurpose Technology for a Wide Spectrum of Global Biosecurity Applications, Journal of the Association for Laboratory Automation, 14: 148 – 156.

 

Detection of Intracellular Granularity Induction in Prostate Cancer Cell Lines by Small Molecules Using the HyperCyt High-Throughput Flow Cytometry System

K. Haynes, J. J.Strouse, A. Waller, A. Leitao, R. F. Curpan, C. Bologa, T. I. Oprea, E. R. Prossnitz, B. S. Edwards, L. A. Sklar, and T. A. Thompson. (2009). Detection of Intracellular Granularity Induction in Prostate Cancer Cell Lines by Small Molecules Using the HyperCyt High-Throughput Flow Cytometry System, J Biomol Screen, 14: 596 – 609.

 

 

Year: 2008

Screening for modulators of androgen receptor transcriptional activity using a high-throughput, flow-cytometry system

Dennis, H. Bowles, L. Sklar, E. Prossnitz, and T. Thompson. (2008). Screening for modulators of androgen receptor transcriptional activity using a high-throughput, flow-cytometry system, AACR Meeting Abstracts, 2008: LB-150.

 

High-Throughput Screening for Daunorubicin-Mediated Drug Resistance Identifies Mometasone Furoate as a Novel ABCB1-Reversal Agent

S. Winter, D. M. Lovato, H. M. Khawaja, B. S. Edwards, I. D. Steele, S. M. Young, T. I. Oprea, L. A. Sklar, and Richard S. Larson. (2008). High-Throughput Screening for Daunorubicin-Mediated Drug Resistance Identifies Mometasone Furoate as a Novel ABCB1-Reversal Agent, J Biomol Screen, 13: 185 – 193.

 

 

Year: 2007

Identification of Small-Molecule Inhibitors of RGS4 Using a High-Throughput Flow Cytometry Protein Interaction Assay

L. Roman, J. N. Talbot, R. A. Roof, R. K. Sunahara, J. R. Traynor, and R. R. Neubig. (2007). Identification of Small-Molecule Inhibitors of RGS4 Using a High-Throughput Flow Cytometry Protein Interaction Assay, Mol. Pharmacol, 71: 169 – 175.

 

 

Year: 2005

High-Throughput Screening with HyperCyt Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands

M. Young, C. Bologa, E. R. Prossnitz, T. I. Oprea, L. A. Sklar, and B. S. Edwards. (2005). High-Throughput Screening with HyperCyt Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands, J Biomol Screen, 10: 374 – 382.

 

Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists

S. Edwards, C. Bologa, S. M. Young, K. V. Balakin, E. R. Prossnitz, N. P. Savchuck, L. A. Sklar, and T. I. (2005). Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists, Oprea. Mol. Pharmacol., 68: 1301 – 1310.

 

 

Year: 2004

High-Throughput Microfluidic Mixing and Multiparametric Cell Sorting for Bioactive Compound Screening

M. Young, M. S. Curry, J. T. Ransom, J. A. Ballesteros, E. R. Prossnitz, L. A. Sklar, and B. S. Edwards. (2004). High-Throughput Microfluidic Mixing and Multiparametric Cell Sorting for Bioactive Compound Screening, J. Biomol Screen, 9: 103 – 111.

 

Real-time Analysis of Ternary Complex on Particles: DIRECT EVIDENCE FOR PARTIAL AGONISM AT THE AGONIST-RECEPTOR-G PROTEIN COMPLEX ASSEMBLY STEP OF SIGNAL TRANSDUCTION

C. Simons, S. M. Biggs, A. Waller, T. Foutz, D. F. Cimino, Q. Guo, R. R. Neubig, W. Tang, E. R. Prossnitz, and L. A. Sklar. (2004). Real-time Analysis of Ternary Complex on Particles: Direct Evidence for Partial Agonism at the Agonist-Receptor-G Protein Complex Assembly Step of Signal Transduction, J. Biol. Chem., 279: 13514 – 13521.

 

 

Year: 2003

Ligand-Receptor-G-Protein Molecular Assemblies on Beads for Mechanistic Studies and Screening by Flow Cytometr

C. Simons, M. Shi, T. Foutz, D. F. Cimino, J. Lewis, T. Buranda, W. K. Lim, R. R. Neubig, W. E. McIntire, J. Garrison, E. Prossnitz, and L. A. Sklar. (2003). Ligand-Receptor-G-Protein Molecular Assemblies on Beads for Mechanistic Studies and Screening by Flow Cytometry, Mol. Pharmacol., 64: 1227.

 

Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium falciparum Infected Erythrocytes Using Flow Cytometry

Teo , W. Hasang, S. Rogerson, M. Vaccines. Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium falciparum Infected Erythrocytes Using Flow Cytometry, Volume 1325 of the series Methods in Molecular Biology, pp 207-213.

 

A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium falciparum-Infected Erythrocyte

Teo , W. Hasang, P. Boeuf, S. Rogerson, Malaria Vaccines. A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium falciparum-Infected Erythrocytes, Volume 1325 of the series Methods in Molecular Biology, pp 145-152.

 

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