iQue® Human General Immune Cell Killing Kit

Key Benefits

• Speed: Expedient sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
• Results: Five endpoints from a single well assay measure target and effector cells, mitochondrial membrane depolarization, cell membrane integrity, cell count, and secreted pro-apoptotic protease Granzyme B.
• Biological insights: Investigation of multiple mechanisms of immune cell mediated cell killing in one well.
• Ease of use: Single platform assay and data analysis with iQue Forecyt® eliminates the time required for acquiring and correlating data from multiple sources.

Kit Description

The iQue® Human General Immune Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye and mitochondrial membrane potential dye.
• Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
• Simultaneous analysis of multiple mechanisms of immune cell mediated cell killing in a single, high content assay including mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, and pro-apoptotic protease Granzyme B.

Workflow

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