iQue® Human NK Cell Killing Kit

Key Benefits

• Speed: Sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
• Results: Nine endpoints from a single well assay measuring activation markers (CD69 and CD25), cell membrane integrity, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic protease Granzyme B.
• Biological insights: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
• Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.
• Flexibility: Choose additional cytokine measurements from a validated list of iQue® Human NK Cell Companion Kits.

Kit Description

The iQue® Human NK Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
• Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
• NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells. The expression of the IgG Fc receptor III (CD16) on the NK cells can then be assessed.
• The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
• Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Workflow

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iQue® Human NK Cell Companion Kits

The iQue® Human NK Cell Companion Kits may be used in combination with the iQue® Human NK Cell Killing Kit to allow measurement of up to 6 additional human cytokines/effector proteins along with IFNγ and Granzyme B which are already included in the iQue® Human NK Cell Killing Kit.