iQue® Human T Cell Exhaustion Kit

Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 11 endpoints from a single-well assay measuring T cell exhaustion phenotypes and function, as well as cytokine secretion in response to stimulation, cell viability, and an optional proliferation dye allows screening for molecules that reverse T cell exhaustion.
• Biological insight: Generate functional data and analyze cell health from the same well by multiplexing cell and cytokine detection in one assay.
• Simplicity: Single platform assay and data acquisition and analysis eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The iQue® Human T Cell Exhaustion Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:

• Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.
• The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.
• Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.
• The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).

Workflow

Request a Quote