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Intellicyt® Antibody Internalization Kits and Reagents

February 27, 2019

Key Benefits

  • No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization.
  • High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 µL of cells and 10 µL of labeled antibody (kit).
  • Simultaneously measure antibody internalization, cell specificity, and cell health within the same well.
  • Available in Human and Mouse species.

Kit Description

The Intellicyt Multiplex Antibody Internalization Kits are high throughput, multiplexed, no-wash assays that measures antibody internalization, cell specificity, and cell health using 10 µL sample of cells and 10 µL antibody. Available in 1 x 96, 5 x 96, 1 x 384 and 5 x 384-well format.

The Intellicyt Antibody Internalization Reagents are designed to measure internalization of a large number of antibodies in 96- and 384-well formats using 20 µL of cells and 20 µL of labeled antibody.

Principle

Antibodies are easily labeled with Intellicyt Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.

Cell viability can also be measured to assess general cell health and antibody function. Using the same sample, cell specificity can be characterized using encoding dyes (cell populations/types). Data acquisition is performed using the iQue3 and ForeCyt® software for data analysis and visualization.

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Product NameAvailable SizesCatalog No
Intellicyt® Antibody Internalization Human Reagent1 x 9690564
1 x 38490565
Intellicyt® Antibody Internalization Mouse Reagent1 x 9690566
1 x 38490567
Intellicyt® Multiplex Human Antibody Internalization Kit1 x 9697046
5 x 9697047
1 x 38497048
5 x 38497049
Intellicyt® Multiplex Mouse Antibody Internalization Kit1 x 9697050
5 x 9697051
1 x 38497052
5 x 38497053

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