- No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization.
- High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 µL of cells and 10 µL of labeled antibody (kit).
- Simultaneously measure antibody internalization, cell specificity, and cell health within the same well.
- Available in Human and Mouse species.
The iQue® Multiplex Antibody Internalization Kits are high throughput, multiplexed, no-wash assays that measures antibody internalization, cell specificity, and cell health using 10 µL sample of cells and 10 µL antibody. Available in 1 x 96, 5 x 96, 1 x 384 and 5 x 384-well format.
The iQue® Antibody Internalization Reagents are designed to measure internalization of a large number of antibodies in 96- and 384-well formats using 20 µL of cells and 20 µL of labeled antibody.
Antibodies are easily labeled with iQue® Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.
Cell viability can also be measured to assess general cell health and antibody function. Using the same sample, cell specificity can be characterized using encoding dyes (cell populations/types). Data acquisition is performed using the iQue® 3 and iQue® Forecyt® software for data analysis and visualization.
|Product Name||Available Sizes||Catalog No|
|iQue® Human Antibody Internalization Reagent||1 x 96||90564|
|1 x 384||90565|
|iQue® Mouse Antibody Internalization Reagent||1 x 96||90566|
|1 x 384||90567|
|iQue® Human Multiplex Antibody Internalization Kit||1 x 96||97046|
|5 x 96||97047|
|1 x 384||97048|
|5 x 384||97049|
|iQue® Mouse Multiplex Antibody Internalization Kit||1 x 96||97050|
|5 x 96||97051|
|1 x 384||97052|
|5 x 384||97053|