iQue® Human 4-Plex Apoptosis Kit
January 05, 2016
Key Benefits
- No wash protocol – just add and read for fast, easily automated workflow. Our iQue® Human 4-plex kit is easier to run than some single endpoint kits!
- Four parameters to deliver rich content, including insight on mechanism of action.
- The 4 components are available separately so you can pick and choose.
- Multiplex with iQue Qbeads® PlexScreen kits for richer content.
Kit Description
The iQue® Human 4-Plex Apoptosis Kit is a multi-parameter assay consisting 4 different apoptosis measurements per well (cell viability, caspase 3/7 activity, annexin V binding, and mitochondrial depolarization) for a robust determination of apoptosis progression and insight into mechanism of action. The individual reagent components are also available separately for greater flexibility.
Principle
The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.
Surface expression of phosphatidylserine is detected by the binding of Annexin-V to the cell surface.
Cell viability is determined by the ability of live cells to exclude a DNA binding.
Mitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.