Antibody internalization and associated kinetics are critical characteristics that influence the mechanism of action of antibody-based drugs. During antibody-based drug discovery, modifications made to increase biological potency can have profound effects on antibody activity and specificity, as well as antibody internalization.

This necessitates thorough in vitro, high throughput screening of antibodies during the early discovery process to quickly identify the most suitable drug candidates for further development.

This application note highlights the ability of the Intellicyt® iQue Screener Plus platform to quickly profile and compare large sets of antibodies and characterize their effects on targets for key attributes, such as antibody internalization, within the same sample.