Application Note: An Optimized, Multiplexed Assay for Screening Ex Vivo Conditions which Increase Memory T Cell Frequency

Ex vivo expansion of T cells is a critical process in bio-manufacturing of adoptive cell therapies such as chimeric antigen receptor (CAR) T and tumor infiltrating lymphocyte (TIL) therapies

Recent clinical studies suggest that ex vivo T cell expansion protocols generating higher percentages of T memory stem cells (Tscm), central memory T cells (Tcm) in the total cell product are critical to significant clinical improvements in adoptive cell therapies.

Download this application note to learn how the need to monitor T cell phenotype and function for improved T cell expansion protocols was addressed with a T Cell Memory Cell and Cytokine Profiling assay that enabled measuring memory cell phenotype, cell viability and effector cytokine release in the same sample well in a miniaturized assay format.