Tutorial: AAI 2016 Massachusetts General Hospital : Using the iQue Screener for high throughput screening of small molecules and CRISPRs in a system of myeloid progenitors

This tutorial describes how researchers at Massachusetts General Hospital used the Intellicyt platform to conduct a high throughput functional screen (leukemia screen) of small molecules to evaluate their therapeutic potential in Acute Myeloid Leukemia (AML).

This tutorial highlights the ability of the iQue Screener to:

  • Perform phenotypic screening of large compound libraries for cell function
  • Perform target identification and validation studies with genetic permutation screens like CRISPR
  • Measure differentiation, viability and apoptosis as well as cytokine release over a time continuum

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