SLAS 2015 Sharp Edge Labs: Speed and Sensitivity – The Special Advantages of High Throughput Cytometry for Cell Surface Protein Trafficking Assays

Protein trafficking is critical to many biological processes, and is defective in many well-known diseases including Cystic Fibrosis. Cell-surface protein trafficking is especially important in health and disease because it effects surface density, and thus receptor/transporter/channel activity

This tutorial will overview:

• The use of the Fluorogen Activating Peptide technology for measuring protein trafficking of cell-surface proteins, particularly CFTR (the Cystic Fibrosis Chloride Channel) and P-glycoprotein (PgP, a related drug-pump/MDR protein.
• The development of a high-throughput screening assay using the iQue Screener to screen for compounds capable of CFTR restoration (as possible treatments in Cystic Fibrosis)
• Advantages offered by the iQue Screener in comparison to imaging