Antibody Screening

Visualization of Screen Results

Results from the entire screen visualized using the Panorama feature in ForeCyt. 3 cell lines were color coded with the fluorescent Cell Encoder dye mixed together and dispensed into wells of 384 well plates. Hybridoma supernatants were added to each well, followed by a fluorescent detection antibody. 9,600 hybridomas were tested from 2 different mice immunized with cells expressing target antigen. The entire screen was completed in 1 day.

Heat maps (red) showing results for antibody binding to control cells, cells expressing target antigen and cells expressing a related but irrelevant antigen.

Profile maps (blue) combine the binding results to the three cell types, showing antibodies that bind to cells expressing the target antigen, but not to cells expressing an irrelevant antigen or to negative control cells.