9600 hybridoma clones screened from 2 different immunized mice.
25 x 384 well plates run and analyzed in 1 day.
Multiplex cell based assay enables single-step screening for antibodies that bind to target antigens but not other antigens.
28,800 data points instantly combined and processed into binding metrics.
53 hits visualized that bind to target but not control antigens.
Results from the entire screen visualized using the Panorama feature in…
Results from the entire screen visualized using the Panorama feature in ForeCyt. 3 cell lines were color coded with the fluorescent Cell Encoder dye mixed together and dispensed into wells of 384 well plates. Hybridoma supernatants were added to each well, followed by a fluorescent detection antibody. 9,600 hybridomas were tested from 2 different mice immunized with cells expressing target antigen. The entire screen was completed in 1 day.
Heat maps (red) showing results for antibody binding to control cells, cells expressing target antigen and cells expressing a related but irrelevant antigen.
Profile maps (blue) combine the binding results to the three cell types, showing antibodies that bind to cells expressing the target antigen, but not to cells expressing an irrelevant antigen or to negative control cells.
Select antibody clones with desired characteristics using the Profile Maps feature on ForeCyt Software.
Learn how scientists and ModiQuest Research incorporated the iQue Advanced Flow Cytometry Platform across different stages of their antibody discovery workflow.