Successful antibody discovery programs need to rapidly identify and characterize target-reactive candidates. This case study demonstrates how the Intellicyt® iQue Screener platform can be used for target cell line generation, mouse sera evaluation and hybridoma library screening.

Hybridoma Library Screening

A high throughput, multiplexed screen that simultaneously tests for binding to target antigens but not control antigens. The entire screen and data analysis were completed in one day.

Antibody plates

Increase Productivity

9600 hybridoma clones screened from 2 different immunized mice.

25 x 384 well plates run and analyzed in 1 day.

Powerful, Multiplexed Screening Assay

Multiplex cell based assay enables single-step screening for antibodies that bind to target antigens but not other antigens.

Fast and Easy Visualization of Results

28,800 data points instantly combined and processed into binding metrics.

53 hits visualized that bind to target but not control antigens.

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Visualization of Screen Results

Results from the entire screen visualized using the Panorama feature in…

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iQue Screener Advantages

Select antibody clones with desired characteristics using the Profile Maps feature on ForeCyt Software.

Download Application Note

Learn how scientists and ModiQuest Research incorporated the Intellicyt® iQue Screener across different stages of their antibody discovery workflow.

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