Successful antibody discovery programs need to rapidly identify and characterize target-reactive candidates. This case study demonstrates how the Intellicyt® iQue Screener platform can be used for target cell line generation, mouse sera evaluation and hybridoma library screening.

Mouse Sera Evaluation

Large scale cell based experiment to identify immunized mice producing high levels of antibody against target antigens. Simultaneously testing sera from dozens of immunized mice improves chance of successfully generating hybridomas with optimal antibody reactivity

Antibody plates

Increase Productivity

Sera from 36 immunized mice tested for binding to cell surface target antigens.

Sera from mice pre- and post-immunization were tested in 8 fold dilutions in duplicate

Powerful, Multiplexed Screening Assay

Multiplex cell based assay enables single-step screening for sera that bind to target antigens but not other antigens.

Visualization of Results

Fast and Easy Visualization of Results

Use heat maps to visualize mouse sera that have high titers of antibodies that bind to target antigens but not other antigens.

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Visualization of Screen Results

Results from the testing visualized using the HeatMap feature in ForeCyt…

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iQue Screener Advantages

Simultaneously test sera from multiple immunized mice.

Download Application Note

Learn how scientists and ModiQuest Research incorporated the Intellicyt® iQue Screener across different stages of their antibody discovery workflow.

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