Antibody Screening
Successful antibody discovery programs need to rapidly identify and characterize target-reactive candidates. This case study demonstrates how the iQue Advanced Flow Cytometry Platform can be used for target cell line generation, mouse sera evaluation and hybridoma library screening.
Mouse Sera Evaluation
Large scale cell based experiment to identify immunized mice producing high levels of antibody against target antigens. Simultaneously testing sera from dozens of immunized mice improves chance of successfully generating hybridomas with optimal antibody reactivity
Increase Productivity
Sera from 36 immunized mice tested for binding to cell surface target antigens.
Sera from mice pre- and post-immunization were tested in 8 fold dilutions in duplicate
Powerful, Multiplexed Screening Assay
Multiplex cell based assay enables single-step screening for sera that bind to target antigens but not other antigens.
Fast and Easy Visualization of Results
Use heat maps to visualize mouse sera that have high titers of antibodies that bind to target antigens but not other antigens.
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Visualization of Screen Results
Results from the testing visualized using the HeatMap feature in ForeCyt…
iQue Advanced Flow Cytometry Platform Advantages
Simultaneously test sera from multiple immunized mice.
Download Application Note
Learn how scientists and ModiQuest Research incorporated the iQue Advanced Flow Cytometry Platform across different stages of their antibody discovery workflow.
